10X PBS SIGMA

Aug 21, 11
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  • Below is the recipe for 10x PBS. Dissolve all ingredients in 800 mL . Or even easier: Just use pre-made 10x PBS (Sigma 79383), or tablets (Sigma P4417). .
  • 353219); 10X Phosphate Buffered Saline (PBS): Prepare a 1X PBS solution by . the 10X PBS stock with DI water; 37% Formaldehyde solution (Sigma-Aldrich .
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  • Known as D-PBS, this is a general use, isotonic saline solution for washing cells and tissues. D 1283, Dulbecco's Phosphate Buffered Saline (10x) .
  • 10x PBS, pH 7.0 (200mM KPO4, 140 mM NaCl) 500 mM EGTA pH 7.4 37 .
  • BSA (Sigma Cat # A-7030); Avidin-HRP conjugate (Sigma cat. # A-7419); ABTS Liquid Substrate Solution (Sigma Cat. # A3219); Dulbecco's PBS [10x] (Gibco BRL .
  • PBS, 10X Solution, pH 7.4. Affymetrix / USB, 100 ml, 10 X . . Sigma-Aldrich; Spring Bioscience; Thermo Scientific Pierce Protein Research Products .
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  • 10x PBS (Dulbecco's phosphate buffered saline) with 100µM thimerosal. Slowly add 96.5g of Sigma D-5773 to 950ml of filtered water while stirring .
  • Tween 20: Sigma, P7949. 10X PBS, pH 7.4: Fisher Cat.# BP399-4. 20X LumiGLO® Reagent and 20X Peroxide, Cell Signaling Technology Cat. #7003. Equipment: .
  • 10X PBS. WFID: 10136 Date: 06-27-2011. Protocol Format: Public Draft. Morassa Mohseni . 3, S5136, Na2HPO4, Sigma, Solute. 4, P9791, KH2PO4, Sigma, Solute .
  • NOTE: This is to make stock 10X PBS. 1X PBS, as needed for DAPI, is located below. . NaCl (Fisher, BP358-212) - 80g; KCl (Sigma, P9541-500G) - 2.0g .
  • 0.41 g (2 mM) MgCl2. 0.02% NP40 (diluted from a 10% stock). 0.01 .
  • Lysing solution: 10% Triton X-100 (Sigma) . cat# 50-61-01) (2) 10 ml 10X PBS ( Dulbecco's without Ca and Mg) (3) 0.2 ml Tween 20 (Sigma Cat# P-1379) (79,8 .
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  • Known as D-PBS, this is a general use, isotonic saline solution for washing .
  • 10X PBS. Phosphate buffered saline, recipe from Harlow and Lane. . . Suspend 10 g of diatomaceous earth (Sigma D-5384) in 100 ml of distilled water in a .
  • Enjoy 40% #discount on #10X PBS from sigma, check www.scienceoffers.com for detail. 7:48 AM Jun 15th via Twitter for iPhone .
  • 10x PBS Recipe for 1 liter. Dissolve in 800 ml distilled water: . 0.5ml Tween 20 {Sigma No.:P-1379, Polyoxylethylene-sorbitan monolaurate (Tween 20)} .
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  • All, BioShop, Gibco, Sigma-Aldrich. Next 1 to 10 of 19 . PBS, 10X. pH 7.4, 4L (BioShop). View Detail, Add to Order .
  • Prepare a 10X PBS stock solution. • Prepare a stock solution of 20%(w/v) n- propyl gallate (Sigma P3130) in dimethyl formamide or dimethyl sulfoxide. .
  • sodium phosphate monobasic (Sigma, cat. #S-0751) . 10X PBS (6mM Na Phosphate monobasic, 71mM Na Phosphate dibasic, 1.4M NaCl) .
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  • The cells are washed 4 times for 10 minutes with 1x PBS and then mounted in .
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  • 10x PBS, pH 7.0 (200mM KPO4, 140 mM NaCl) 500 mM EGTA pH 7.4 37% formaldehyde ( Sigma), 10x PBS Triton X-100. BSA, 10x PBS Triton X-100 .
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  • Resuspend the cell pellet in 1 ml of phosphate buffered saline (PBS) and repellet by . The Master mix buffer is often stored as a 10X stock solution ( 100 mM . N = # of primer bases; SIGMA 260 =~ 10000 X N/ m X cm; Molecular weight .
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  • ml Teleostein Gelatin ∼45% in ddH2O (Sigma Cat# G7765). 0.5. ml 20% Triton X- 100. 10. ml 10X PBS. 0.1. g Casein (Enzymatic Hydrolysate) *OPTIONAL* .
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  • 0.01% Deoxycholic Acid Sodium Salt (Sigma #D-6750). 50 ml 10X PBS. up to 500 ml with Q. store at 4°C in a light proof glass bottle. 100X X-Gal. 1 g X-gal .
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  • PBS, 10x Powder Concentrate (White Granular Powder). Fisher BioReagents, 2x1 .

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