Measurement of gene expression by translational coupling: effect of copy

Jul 8, 1994 . However, there is no fixed stoichiometry of coupling: mutations in atpH lead to

Although acting originally on the expression of only one of the two main coupling

This mutation decreases rpml expression about 10-fold and also decreases that

Cell Free Expression; Translation Systems; "Linked" & "Coupled" Systems .

Measurement of gene expression by translational coupling: effect of copy

Mutational alterations of translational coupling in the L11 .

Effects of SDBb and SDBc mutations on expression of β-galactosidase from the

Translational coupling is the mechanism by which an appropriate quantitative .

The nature of translational coupling between repB and repA, the overlapping rep

assay for translational coupling, the promoter-distal alpEgene was fused to lacZ,

translational coupling (Fig. 4). Q 1999 Blackwell Science Ltd, Molecular

Several mutational analyses were carried out to define the region responsible for

to impact the RNA metabolism pathway at multiple levels from transcrip- tion to

Mutations that disrupt the long-range interaction abolish the translational

laps the pyrF translational stop codon, suggesting that the two genes are

translation should abolish nutR function. Alternatively, translational coupling may

cite evidence that translational coupling is involved in the regulation of several

sr575 mutation, leading to an increased rate of lyso- zyme synthesis. While such

It has been specifically postulated that a translational coupling at the nut site is

Changes in the survival of each mutant indicate a role of the mutated region . .

Overlap is most common in rapidly evolving genomes with high mutation rates .

May 11, 2010 . In one examined case we confirmed that the second site mutations had restored

Translation of repA and repB is likely to be coupled in pSW800 since mutation of

Restoration of a translational stop-start overlap reinstates translational coupling

Oct 1, 1982 . A Founder Mutation in the GK1 Gene Is Responsible for Galactokinase .

In one examined case we confirmed that the second site mutations had restored

Restoration of a translational stop-start overlap reinstates translational coupling

Praszkier J. Mutations affecting translational coupling between the rep genes of

Publication » Measurement of gene expression by translational coupling: effect of

Translational coupling in the Escherichia coli operon encoding translation

ing the principle of translational coupling was investigat- ed. For this purpose, the

translational coupling may not be involved in N action; how- ever, the nusE

Jun 14, 2011 . Measurement of gene expression by translational coupling: effect of copy

Emergence of the mutant phenotype via coupled transcription/translation will be

Translational coupling in the Escherichia coli operon encoding translation .

Nov 19, 2010 . A. To test for translational coupling, site-directed mutation was used to introduce

reinitiation is an extreme case of translational coupling in which translation of a

A. To test for translational coupling, site-directed mutation was used to introduce

Jul 2, 2004 . [Note -- DeLucia and Cairns' isolated a polA mutant that was not conditional, .

The effect of a putative translational enhancer sequence (downstream box) on

Jun 9, 2006 . test the possibility of translational coupling, a stop codon. (UAA) was . .. overlap

Effects of SDBb and SDBc mutations on expression of ß-ga- lactosidase from the

and functional properties of the Jk mutant polypeptide. In the cell-free

The nature of translational coupling between repB and repA, the overlapping rep

Translational Overlap and Translational Coupling . . . The ampC attenuator

that the mRNA contains functionally distinct ribosome and repressor binding sites

TRANSLATIONAL COUPLING MUTATIONS. 4687. TABLE 1. Bacterial strains.

Aug 16, 2011 . To exclude the possibility that egfp interferes with translational coupling, we

least three times more efficiently than atpH. However, there is no fixed

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