comparison with other published laccase protein sequences) to be towards the.

2.2. Cloning of A. gambiae and M. sexta laccase. cDNAs. Degenerate primers

Using degenerate PCR primers based on conserved regions within the copper-

A laccase from Panus rudis was produced constitutively in defined shaken . .

Jun 27, 2006 . In particular, laccase is known to be associated with lignin synthesis (reviewed . .

The amino acid sequences that are used to design the degenerate primers are

We used the well working primer combination Cu1F & Cu2R, as well as the new

The laccase (benzenediol:oxygen oxidoreductase, EC 1.10.3.2) gene from .

Anchored PCR was applied to clone the laccase gene fragment based on the

Degenerate primers corresponding to the consensus sequences of the copper-

Aug 9, 2005 . Insects injected with dsRNA for the laccase 2 gene failed to tan, were . . The

A degenerate PCR-primer pair Cu1F/Cu2R, specific for basidiomycetes, was

Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by

SpringerImages - Primers used for qRT-PCR of laccase and manganese

A new laccase gene (lacC) was cloned from the genomic DNA isolated from

Apr 19, 2011 . The present invention relates to a novel laccase enzyme obtainable . of the PCR

a laccase gene sequence, suggesting the specificity of the primers. PCR

The diversity of ascomycete laccase sequences was surveyed in a southeastern

Identification of laccase-producing basidiomycete and xylariaceous fungi using

Nov 17, 2006 . Fungal laccases generally have four conserved copper-binding regions, and the

Laccase Gene LAC1 of Colletotrichum lagenarium Is Not Essential for . chain

Strategy used for PCR cloning of the laccase-encoding cDNA from P.

and the Primer 3 free software. These primers were used to amplify different

manufacturer's instructions. The cDNA was amplified using laccase gene (

Oct 8, 2011 . was carried out using 12 laccase specific primers (Table. 1). The calibrator used

To obtain laccase-gene-specific sequences from the white-rot fungus Trametes

When we analyzed the 60 pools of the library with primers specific for the seven

Laccase activity was detected in all the fungus combs examined as well as in the

A database of salt marsh ascomycete partial laccase sequences was created

basidiomycete-specific ITS primers for identification. mRNA of laccase enzyme

poor expression of actinomycete genes in E. coli. Laccase-specific primers were

To obtain laccase-gene-specific sequences from the white-rot fungus Trametes

The nt sequence of lcc3 was determined on both strands by primer walking (

As a result of this nonspecificity, these primers should amplify lcc sequences

The laccase-positive SV and CRS-1 isolates were further amplified by

Two specific primer pairs were designed that produced PCR fragments only for

At5g01040 encodes a putative laccase in Arabidopsis. Primers were designed

MR 2. Laccase gene of 300bp was detected in. Morchella sp. MR 2 using Cu1A F

Laccase, an oxidoreductive enzyme is important in bioremediation. . . universal

The sequence of L. tigrinus blue laccase gene lacks some 3' end nucleotides as

Jan 20, 2012 . A highly efficient laccase-producing fungus was isolated from soil and . using

Ectomycorrhizal (ECM) fungi were screened for laccase-like genes by

Genomic clones of laccase were isolated using G. lucidum DNA as a template

Primers used for amplification of miR397, miR857, and ACT2 are shown in Table

used PCR primers targeting laccase (polyphenol oxidase) and quantitative

Four primer sets for the cloning of each of four laccase genes were prepared,

endonuclease-restricted DNA reactions, changing the Gene-specific GW primer (

internal standard and primers for a competitive PCR as described by Bogan et al.

These copper binding regions (cbr) are strongly conserved in all laccases (Fig. 2)

Jun 3, 2008 . Useful information was provided in these sequences for design of new primers to

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