Keywords: ABTS; Laccase; Peroxidase; Phenol oxidase activity; Soil . . The

order ABTS and DMP (Table III). Decolorization of Commercial Dyes. The

This laccase was purified 209-fold from culture fluid with a yield of 6.2% using eth

activity with ABTS acting as chromogen yields exceed- ingly low values

In this assay, ABTS is converted to its radical cation by addition of sodium . "

Oct 10, 2011 . Introduction. This protocols are used to screent for and assay laccase activity. .

Laccase Assay Protocol . Prepare fresh solution of 2 mM ABTS using megapure

Characterization of free and immobilized laccase. 2.5.1. Optimum pH and pH

A laccase-encoding gene from the white-rot fungus. Trametes versicolor . were

pounds by laccase in the presence of a mediator, 1- . Proteins were eluted with

Activity was determined with ABTS as the . laccase was investigated, by

Tannic acid interferes with the commonly used laccase-detection assay based on

Detection was performed using a photodiode array detector at 200 to 600 nm.

Dec 1, 1995 . Laccase activity was demonstrated in the concentrated extracellular culture .

and the gels were activity stained with ABTS. Laccase Activity Assay. The activity

Dec 12, 1997 . Enzyme Assays. Phenol oxidase activity was assayed at 25 °C using 2,2′-azino

TEAC values determined using ABTS-+ and DPPH- radical decolourisation .

Dec 20, 2005 . For assaying laccase activity, transformants were cultivated on . Kinetics

straightforward and so assays can be carried out by mycologists with little . ..

Heterologously expressed laccase activity was monitored in colorimetric enzyme

Samples were analysed daily for laccase activity using the ABTS assay and cell

Laccase activity was determined using ABTS as substrate. Laccase was purified

and 80 mM/min, respectively, using ABTS as a substrate. Copper sulfate . .

. only (XWA6.1). b Detection of laccase production using ABTS overlay assay for

Laccase activity was assayed with two different substrates, ABTS and DMP. PPO-

Oct 20, 2008 . optimum pH of these laccases was studied using ABTS as substrate. It was found

In this work we study the interference of tannic acid with the spectrophotometric

The activity of free laccase was determined by the laccase assay with ABTS as

Laccase activity was assayed using. ABTS substrate [13]. The enzyme units were

Heter- ologously expressed laccase activity was monitored in colorimetric

People are interested in using laccases for a variety of purposes. In . enzyme

May 5, 2011 . Direct Spectrophotometric Assay of Laccase Using Diazo Derivatives of . . Stock

Laccase enzyme assay. Extracellur laccase activity was assayed

Spectrophotometric assays of laccase activity were carried out with 1 mM

Enzyme assays. Laccase activity was assayed at room tempera- ture using 2,2-

Laccase activity was detected with syringaldazine and ABTS 100 μM each . The

with laccase-ABTS followed by alkaline extraction (4). These observations .

8.2.2.1 Laccase Assay. 8.2.2.1.1 Using ABTS (Childs & Bardsley, 1975; Niku-

Dilute the laccase sample with enzyme diluent, if necessary (see Note 15). 2.

Sep 1, 2006 . Laccase activity, measured using the substrates ABTS and . For whole thallus

lected by enzyme assay using crude extract obtained . . Laccase activities were

The laccase activity was tested by spectrophotometry at 420 nm with 2,2'-azobis(

Five laccase assays of the culture medium were carried out at approximately 24-

characterize the organism with respect to laccase gene. White rot . . The selected

enzyme. 2.3. Enzyme and protein assay. Laccase activity was measured at 25. ◦.

Aug 20, 2004 . (2004) Terrón et al. Biochimie. Read by researchers in: 100% Biological

spectrophotometric assay with ABTS [2,2 -azinobis(3-ethylbenzathiazoline-6-

3.3.3 E ZYME ASSAY. Laccase activity was estimated spectrophotometrically

Aug 14, 2008 . By the o-dianisidine and guaiacol oxidation assay, only high activity of laccase

Jan 25, 2011 . teins were eluted with a NaCl gradient from 0 to 1 M. Fractions displaying laccase

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