jem.rupress.org/highwire/filestream/102828/. /JEM_20110462_sm.pdfCells were then treated with GolgiStop and anti–IL-2 (2 μg/ml) for 12 h, in the

www.jimmunol.org/highwire/filestream/323352/field_highwire. /s.pdfmice (filled histogram). Supplemental Figure 3. IFN-γ versus IL-22 production

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https://www.researchgate.net/. /To_do_cytokine_staining_in_lymphocyte_eg _IFN-g_are_simutaneous_stimulation_and_Golgi_block_essentialSimilarAssuming your initial activation stimulus is a good IFNg inducer then no, you don'

www.sputtr.com/golgistopCachedKit Manual. The BD Cytofix/Cytoperm ™ Plus Fixation/Permeabilization Kit (with

softflowbio.com/bd-golgistop-protein-transport-inhibitor-(containing- monensin)CachedSimilarBD GolgiStop Protein Transport Inhibitor (containing Monensin). Catalog no.:

www.bd.com/resource.aspx?IDX=17687CachedAfter stimulation of cells in the presence of BD GolgiStop™ protein transport

www.protocol-online.org/biology-forums-2/posts/9881.htmlCachedSimilarWe ( my bench mate and I) tested the PMA/Iono + golgi stop (added at different

https://www.biolegend.com/fr-fr/. /monensin-solution-1-000x-1500CachedSimilarMonensin Solution (1000X) - Monensin is a protein transport inhibitor commonly

www.cell.com/cms/attachment/2097794121/2078425931/mmc1.pdf055:B5) in vitro in combination with GolgiSTOP (1:2000, BD Biosciences).

https://www.bioz.com/search/golgistop%20monensinCachedAfter 1 h incubation, GolgiStop monensin (1000×, Biolegend) was added into

www.nature.com/ni/journal/v12/n6/fig_tab/ni.2030_F1.htmlSimilar(b,c) Analysis of cell division (b) and intracellular staining for IFN-γ (b,c) in splenic

www.molmeth.org/. /simultaneous-detection-murine-antigen-specific- intracellular-cytokines-and-cd107acd107bCachedMar 8, 2013 . Prepare working stock of PMA+Ionomycin activating reagent. 100 μl of working

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. 177, Genetic engineering, 213 Genetic association study, 74 Genetic variation,

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jvi.asm.org/content/88/17/10259.figures-onlyJun 18, 2014 . PBMCs were cultured in the presence of anti-CD28/CD49d, GolgiStop, and

Thereafter, add BD GolgiStop (BD) into the culture. Incubate for 6 h in the

https://www.thermofisher.com/order/catalog/product/00-4980-03CachedThe Protein Transport Inhibitor Cocktail (500X) is a cocktail of Brefeldin A and

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. number of antigen-specific CD4+ precursor cells generated by a DNA vaccine (

There is also a temporal change in the Golgi, for, when a pollen grain germinates

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https://www.researchgate.net/. /What_concentration_of_Golgi_stop_ monensin_for_CD107a_assaySimilarWe are performing a co-culture experiment with very few NK cells and target cells

https://www.reddit.com/r/. /issues_with_intracellular_staining/CachedSimilarTry golgiplug or golgistop. The active ingredients in both blocks secretion of

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www.springerprotocols.com/cdp/search/searchResultPage?text. SimilarResults 1 - 10 of 263 . Summary: , in the presence of a protein transport inhibitor such as Brefeldin A or

www.kumc.edu/. /Intracellular%20Staining%20Methods%20and%20Notes. pdfCachedSimilarinhibitors, Cytofix/Cytoperm Plus Kit with BD GolgiStop™ protein transport

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Block of cytokine secretion and intracellular accumulation is achieved by

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2922400/Jun 12, 2010 . GolgiStop (active ingredient monensin; Becton-Dickinson, San Diego, CA) or

www.ispybio.com/search/protocols/fc%20protocol112.pdfCachedSimilarFor Flow cytometry analysis we block the secretion of the cytokines with: –

journals.plos.org/plosone/article/figures?id=10.1371/journal.pone. PBMC from patients before KT, patients at 1month after KT and patients at 3

www.ucan-u.org/. /Immunofluorescent_staining_and_flow_cytometric_ analysis_of_cells.pdfCachedSimilarThe analysis of single cells in this way is called flow cytometry and the

hiv-forschung.de/wp-content/. /NK-DEGRANULATION-ASSAY.pdfCachedInstitut für HIV Forschung. NK Cell Degranulation Assay. Reagents. Reagent.

clincancerres.aacrjournals.org/highwire/. /CCR-12-2422methfig1-.pdfL of Golgi Stop (monesin, BD). All tubes were incubated at 37°C for 4 hours and

https://www.bdbiosciences.com/us/. /research/. /554724CachedBrand: BD GolgiStop™; Contents: Monensin solution; Application: Intracellular

ulab360.com/files/prod/manuals/201504/16/134555001.pdfCached554714) as well as the BD Cytofix/Cytoperm Plus Kit with BD GolgiStop™ protein

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