press.endocrine.org/doi/pdf/10.1210/endo.139.8.6139Similarand Two Glucagon-Like Peptides with Insulin-Releasing. Activity from the . .. J,

www.biochemj.org/content/440/3/397Dec 15, 2011 . More than 20 years after the description of the two IR (insulin . . [36–39] and

www.chem.uci.edu/~gweiss/Morr-sg-rev.pdfCachedSimilarshotgun scanning uses phage-displayed libraries of alanine- substituted . .

www.jbc.org/content/272/20/12978.abstract?ck=nckAlanine scanning mutagenesis has been used to identify specific side chains of

journals.plos.org/plosone/article?id=10.1371/journal.pone. CachedSimilarOct 30, 2013 . Scanning mutagenesis is a powerful protein engineering technique used to study

journal.frontiersin.org/article/10.3389/fendo.2011.00076/fullCachedOct 31, 2011 . Ever since the discovery of insulin and its role in the regulation of . have been

(1990) Functionally distinct insulin receptors generated by tissue-specific .

authors.library.caltech.edu/3760/1/CHEjbc97.pdfCachedSimilarMannose 6-Phosphate/Insulin-like Growth Factor II Receptor. Cytoplasmic

www.thermalin.com/. /pnas_july_10_2012_alpha-helical_element_at_the_ hormone-binding_surface.pdfCachedJul 10, 2012 . receptor activation. nonstandard mutagenesis | alanine scanning | affinity. Insulin

link.springer.com/article/10.1007/s11030-012-9368-zBinding site characterization of G protein-coupled receptor by alanine-scanning

https://www.ncbi.nlm.nih.gov/pubmed/9148904

onlinelibrary.wiley.com/doi/10.1002/jcc.20512/abstractSep 18, 2006 . Importance of individual side chains for the stability of a protein fold:

www.diapedia.org/. insulin. /insulin-binding-and-activation-of-the-insulin- receptorCachedSimilarSep 30, 2014 . The insulin receptor (IR) is a large, disulphide-linked, glycoprotein . .. (i) alanine

ajpendo.physiology.org/content/278/6/E967SimilarIt should be noted, however, that the significant binding epitopes on IGFs are not

ssecor.people.ua.edu/. /general_comp_endocrinology_white_secor_conlon_ 1999.pdfInsulin and peptides derived from the processing of proglucagon have been . ..

www.nature.com/nrd/journal/v1/n10/full/nrd917.htmlSimilarThe amino acids that have been determined by alanine-scanning mutagenesis to

https://f1000research.com/articles/3-214/v2CachedSep 9, 2014 . Alanine scanning webservers with intuitive user interfaces such as Robetta

pubmedcentralcanada.ca/pmcc/articles/PMC3845378/(A) Sequence of human insulin indicating invariant glycine in B chain (GlyB8; . .

www.pnas.org/content/107/15/6771.full.pdfSimilarApr 13, 2010 . The C-terminal segment of the human insulin receptor α-chain . demonstrated

novonordiskpharmatech.com/how-insulin-and-igf-1-bind-to-their-receptors/CachedFigure 2: Insulin binding site 1 and 2. The two insulin sites that bind to the insulin

https://www.jstor.org/stable/3884348however, it has been proposed that the conformation adopted by insulin in the

https://deepblue.lib.umich.edu/bitstream/handle/2027. /24255_ftp.pdf?. Jan 24, 2013 . ''displaced-CT'' complex, many more insulin–receptor contacts . . Mynarcik DC,

www.uptodate.com/contents/structure-and-function. insulin. /56CachedHigh-affinity insulin binding: insulin interacts with two receptor ligand binding

www.slideshare.net/. /a-comparative-structural-bioinformatics-analysis-of-the -insulin-receptor-famiy-ectodomain-based-on-phylogenetic-informa. CachedMar 6, 2016 . Alanine scanning and other mutagenesis data available for conserved residues

jme.endocrinology-journals.org/content/34/3/685.fullSimilarInsulin-like growth factor binding proteins (IGFBPs) have diverse biological

pubs.acs.org/doi/abs/10.1021/bi990089pSimilarApr 27, 1999 . The bioavailability of insulin-like growth factor I (IGF-I) in the serum and tissues is

peds.oxfordjournals.org/content/13/11/779.fullSimilar(1997) carried out extensive alanine-scanning mutagenesis of insulin and

peds.oxfordjournals.org/content/13/11/779.full.pdfmonomeric insulins, we mutated the seven residues by alanine- of insulin dimer.

The three-dimensional structure of the insulin molecule was solved forty years .

www.sciencedirect.com/science/article/pii/S0014579304003394SimilarMapping of the insulin-like growth factor II binding site of the Type I insulin-like

. the Vibrio cholerae virulence regulator ToxT by scanning alanine mutagenesis.

www.genetics.org/content/170/4/1459The second criterion was not interpreted to exclude alanine-scanning studies,

https://pdfs.semanticscholar.org/. / f7022fc5c21fd7d9ce79bfbd93711f7d43b1.pdfDec 27, 2012 . peptide in insulin intramolecular coevolution and correlated residue . .. from the

www.opazolab.cl/Juan_C._Opazo/Publications. /MBE(2005).pdfCachedInsulin is a conservative molecule among mammals, maintaining both its

www.jimmunol.org/content/165/7/3876.full.pdfAlanine Scanning Mutants of Rat Proinsulin I Show . autologous insulin, rat

Alanine scanning studies of the insulin receptor ectodomain (Fig. . Alanine

https://www.hindawi.com/journals/bmri/2013/713585/Sep 10, 2013 . The results from computational alanine scanning and free energy . .. Site-

cshperspectives.cshlp.org/content/5/3/a008946.longInsulin's actions are mediated by the insulin receptor (InsR), a plasma . Previous

https://en.wikipedia.org/wiki/Alanine_scanningCachedSimilarIn molecular biology, alanine scanning is a technique used to determine the

https://www.ncbi.nlm.nih.gov/pubmed/9148904Alanine scanning mutagenesis has been used to identify specific side chains of insulin which strongly influence binding to the insulin receptor. . In contrast, alanine substitutions at positions GlyB20, ArgB22, and SerA9 resulted in an increase in affinity for the insulin receptor.

www.cell.com/trends/biochemical-sciences/. /S0968-0004(08)00145-XChem. 2008; 283: 21198–21210. 46Williams, P.F. et al. Mapping of an NH2-

www.biotechniques.com/. mutagenesis. /biotechniques-145538.html?. CachedIn this report we describe transposon-directed base-exchange mutagenesis (

www.jbc.org/content/276/47/43980.longAug 10, 2001 . In previous alanine-scanning mutagenesis studies of the L1 domain of the

www.mdpi.com/2077-0375/4/4/730/pdfCachedSimilarOct 10, 2014 . Abstract: Insulin binding to the insulin receptor (IR) is the first key step in initiating

A natural mutation at Asn 15 (to Lys) decreased insulin binding 5-fold,” and the

The role of insulin and neurotrophic factor signaling in brain aging and . . ligand

bmcbioinformatics.biomedcentral.com/articles/10. /1471-2105-10-S12-S3SimilarOct 15, 2009 . Several examples of alanine scanning mutagenesis experiments [1] . .. binding

https://cwru.pure.elsevier.com/. /insulin-and-igf-i-receptor-structure-and- binding-mechanism-2CachedThe insulin and IGF-I receptors are members of the superfamily of receptor

europepmc.org/articles/pmc3845378FULL TEXT Abstract: How insulin binds to its receptor is unknown despite

www.jbc.org/content/272/20/12978.abstractSimilarMay 16, 1997 . Alanine scanning mutagenesis has been used to identify specific side chains of insulin which strongly influence binding to the insulin receptor. . The most striking finding is that B20Ala insulin retains high affinity binding to the receptor.

Ishizaki H. and Suzuki A. "An insect brain peptide as a member of insulin family.

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