Filter sterilize the solution with a 0.22 m filter. Store the stock solution at –20ºC.

25 mg/ml vitamin B12 (PASM-5052). 1000X trace metals mix (PA-0.5g, PASM-.

Ampicillin-stock solution (1000×): 100 mg/ml in H2O – Glucose stock solution: .

Antiobiotic Stock solution. Ampicillin (sodium salt): 25 mg/mL in ddH2O. Store at -

1000x Ampicillin stock solution Dissolve 5 g ampicillin in 50 ml of sterile ddH2O.

Chloramphenicol – The frozen stock solutions of chloramphenicol are at 25mg/ml

Filter-sterilize; store at −20◦C • Chloramphenicol (Sigma-Aldrich): 10 mg/ml . and

Chloramphenicol (Sigma): 25 mg/ml stock in ddlTO. Sterilize by filtration . Stock

Rifampicin stock solution: Dissolve 50 mg of Rifampicin in 1 mL DMSO. 6.

Preparation of Stock Solution of Antibiotics Commonly Used 1 . Ampicillin Stock

Ampicillin. Dissolve 50mg 800uL water. Volume to 1mL. Store at –20C. Arginine-

Autoclave and allow the solution to cool up to 55-50C before adding the

Antibiotic stock solutions were kanamycin (25 mg/ml), chloramphenicol (25 mg/ml

Stock Solutions, Media, Reagents and Materials. Item. Concentration / Volume.

1000x Ampicillin stock solution. 1 M Dithiothreitol (DTT). 6x DNA Loading dye.

Dec 20, 2007 . Recipes and stock solutions described in Protein Expression and Purification 41:

Antibiotic Stock solution. Ampicillin (sodium salt): 25 mg/mL in ddH2O. Store at -

Kanamycin 25 mg/ml in water (add 2 ml/L LB = Kan 50) Kanamycin is the . -100

from a fresh plate, never from directly from glycerol stock. If possible . tibiotics as

Prepare a stock solution of 25 mg/ml kanamycin in water and filter-sterilize it into

Jun 29, 2010 . Prepare media for antibiotic resistance experiment a. Kanamycin i. 25 mg/mL. 1.

Prepare fresh solution prior to use. Ampicillin Stock (25 mg/ml). Weigh out 250

4)Chloramphenicol-add 1ml chloramphenicol stock (at 25 mg/ml)per liter of agar

Antibiotic stock solutions: a. 25 mg/mL Rifampicin: dissolve rifampicin in DMSO,

. the cell suspension culture from a stock solution (20 mg/mL) in water-free

Working concentration is 50 μg/ml, or 25 μg/ml for . Stock solution is 35 mg/ml in

The TRV2 and TRV1 vectors both confer kanamycin resistance at 50 ug/mL. . 50

Kanamycin sulfate (Duchefa) stock solution: 100 mg/mL. . Liquid culture medium

Prepare a stock solution of 25 mg/mL kanamycin in H2O and store at 4°C for up

Ampicillin stock solution (25 mg ml-1)(40 ml). Weigh out 1 g ampicillin (sodium

Transfer to a sterile bottle and add appropriate volume of LB in the sterile hood.

Example 1: Suppose you have 3 ml of a stock solution of 100 mg/ml ampicillin (=

For a stock solution of 25 mg/mL: 1 g Ampicillin; 40 mL ddH2O; Filter sterilize.

Jul 9, 2006 . I have to use chloramphenicol in final concentration of 50 ug/ ml in 4 litre of

25mg/ml Kanamycin stock solution. 1. Dissolve 1 g kanamycin sulfate in 35 ml of

100 mg/mL Ampicillin: (1 1000 stock solutions) in dH2O sterilized by filtration

Ampicillin stock solution (25 mg ml-1)(40 ml). 1. Weigh out 1 g ampicillin (sodium

Make 50mg/ml stock = . Do not filter ( the 50% alcohol will sterilise the solution)

5x Phosphate Buffer Stock Solution (0.5 M NaH2PO4;. 50 mM Tris·Cl, pH 8.0) . ..

Antibiotic Stock Solutions. Kanamycin sulfate (Kan) Stock = 25 mg/ml. Make by

Kanamycin stock: 10 mg/ml Ampicillin stock: 100 mg/ml Chloramphicol stock: 25

Example: Suppose you have 3 ml of a stock solution of 100 mg/ml ampicillin (=

Streak out Agrobacterium from glycerol stock onto an LB plate with appropriate .

25mg/ml Ampicillin stock solution recipe. 1. Dissolve 1 g ampicillin (sodium salt)

25mg/ml Ampicillin stock solution. 1. Dissolve 1 g ampicillin (sodium salt) in 35 ml

Recipes for Groves Lab Stocks and Solutions . . Make a 15mg/ml solution of

Preparing solutions for BAC DNA preparation. 1X LB (to . For freezing media,

Chloramphenicol stock soln. (25 mg/ml) 2 ml. Streptomycin stock soln.(5 mg/ml).

Kanamycin. Make up in water to stock solution fo 25mg/ml and sterile filter this is

Feb 19, 2012 . Agar plates 200 mg ml chloramphenicol stock smallest active n-terminal

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